Composite
Truncated

Part:BBa_K4139021:Design

Designed by: Zachary Robinson & Jason Luddu   Group: iGEM22_Lethbridge   (2023-09-27)


Truncated PbEL04


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1391
    Illegal PstI site found at 1517
    Illegal PstI site found at 1640
    Illegal PstI site found at 1763
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal PstI site found at 1391
    Illegal PstI site found at 1517
    Illegal PstI site found at 1640
    Illegal PstI site found at 1763
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1391
    Illegal PstI site found at 1517
    Illegal PstI site found at 1640
    Illegal PstI site found at 1763
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1391
    Illegal PstI site found at 1517
    Illegal PstI site found at 1640
    Illegal PstI site found at 1763
    Illegal AgeI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our antigen of interest tagged with a thioredoxin solubility tag and a His-tag for purification purposes. PbEL04 was truncated for the epitope of interest as the FL-PbEL04 (BBa_K4139012) had trouble expressing.

Source

Plasmodiophora brassicae

References

Ferreira, A. S., Lopacinski, A., Batista, M., Hiraiwa, P. M., Guimarães, B. G., & Zanchin, N. I. T. (2022). A toolkit for recombinant production of seven human EGF family growth factors in active conformation. Scientific Reports, 12(1). https://doi.org/10.1038/s41598-022-09060-9

Jiang, X., Su, Y., & Wang, M. (2022). A small cysteine-rich protein identified from the Proteome of clubroot pathogen, Plasmodiophora brassicae, induces cell death in nonhost plants and host plants. https://doi.org/10.21203/rs.3.rs-1961445/v1